The Human Immune Monitoring Center (HIMC) is seeking a highly motivated Immunoassay/genomics specialist (Life Science Research Professional 2) to join our Immunoassay/Genomics group to perform multidisciplinary projects in human disease. HIMC Immunoassay/Genomics Specialist (LSRP2) Our assays have all been optimized for use with cryopreserved PBMC use with fresh samples must be coordinated in advance. We offer standardized immunophenotyping, phosphoepitope, and intracellular cytokine assays for human blood cells. We have standardized panels for immunophenotyping, phenotyping+intracellular cytokine staining (ICS), and phenotyping+phospho-CyTOF, each with 30 to 38 antibodies.įor immunology-related projects, the HIMC offers full-service RNA and DNA extraction, Bioanalyzer and qubit analysis, BCR/TCR repertoire analysis, microfluidic qPCR arrays, RNAseq library preparation, and BD Rhapsody single-cell Abseq and transcriptomics assays, for human and mouse.įlow cytometry is used to assess immune cell phenotypes and functions. This allows for the combination of many more antibody specificities in a single samples, without significant spillover between channels. Readout is by time-of-flight mass spectrometry. Mass cytometry, or CyTOF ( Fluidigm), is a variation of flow cytometry in which antibodies are labeled with heavy metal ion tags rather than fluorochromes. Custom Luminex panels can be tailored or selected from multiple vendors (Thermo Fisher, EMD Millipore, R&D systems/Biotechne, Bio-Rad). We measure multiple cytokines simultaneously using Luminex bead arrays with up to 80 biomarkers or Olink PEA technology with up to 92 biomarkers per panel. We have standardized immunoassays for detection of human, mouse, rat and NHP cytokines, which can be used to analyze serum, plasma, cell culture supernatants, tissue homogenates, urine, CSF, lavages or other fluids. In partnership with the research community, we also work to test and develop new technologies for immune monitoring. HIMC provides standardized, state-of-the-art immune monitoring assays at the RNA, protein, and cellular level, as well as archiving, reporting, and data mining support for clinical and translational studies. NEWS: The Immune Monitoring Virtual Course happened on September 16th 2020.
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